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Journal of Clinical Microbiology, March 2004, p. 1373-1375, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1373-1375.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Laboratory Confirmation of Generalized Vaccinia following Smallpox Vaccination

Cassandra D. Kelly,1 Christina Egan,1 Stephen W. Davis,1 William A. Samsonoff,1 Kimberlee A. Musser,1 Peter Drabkin,2 James R. Miller,2 Jill Taylor,1 and Nick M. Cirino1*

Wadsworth Center,1 Division of Epidemiology, New York State Department of Health, Albany, New York2

Received 22 August 2003/ Returned for modification 27 October 2003/ Accepted 24 November 2003

The reinitiation of smallpox vaccination has renewed interest in implementing modern diagnostic methods to assess orthopoxvirus infection and adverse events following vaccination. We report here the laboratory confirmation of vaccinia virus in pustular lesions of a healthy adult vaccinee by use of a two-tier algorithm incorporating TaqMan PCR and electron microscopy.

* Corresponding author. Mailing address: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY 12208. Phone: (518) 474-1838. Fax: (518) 486-7971. E-mail: ncirino{at}wadsworth.org.

Journal of Clinical Microbiology, March 2004, p. 1373-1375, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1373-1375.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2004 by the American Society for Microbiology. All rights reserved.