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Journal of Clinical Microbiology, March 2004, p. 981-986, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.981-986.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Real-Time Reverse Transcriptase PCR Assay for Detection of Human Metapneumoviruses from All Known Genetic Lineages

Jeroen Maertzdorf,1 Chiaoyin K. Wang,2 Jennifer B. Brown,2 Joseph D. Quinto,2 Marla Chu,2 Miranda de Graaf,3 Bernadette G. van den Hoogen,3 Richard Spaete,2 Albert D. M. E. Osterhaus,3 and Ron A. M. Fouchier3*

Vironovative BV,1 Department of Virology, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands,3 MedImmune, Mountain View, California2

Received 1 August 2003/ Returned for modification 11 November 2003/ Accepted 26 November 2003

The discovery of human metapneumovirus and its implications for respiratory tract disease have emphasized the need for a sensitive, specific, and rapid assay to detect this virus in a clinical setting. It recently became clear that human metapneumovirus can be grouped into at least four genetic lineages. Previously described assays for the detection of human metapneumovirus were developed by using limited sequence information and failed to detect viruses from all four genetic lineages with comparable sensitivities. Here we describe the development and evaluation of a real-time reverse transcriptase PCR assay that detects human metapneumovirus from the four known genetic lineages with equal specificities and sensitivities.

* Corresponding author. Mailing address: Department of Virology, Erasmus Medical Center Rotterdam, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands. Phone: 31-10-4088066. Fax: 31-10-4089485. E-mail: r.fouchier{at}erasmusmc.nl.

Journal of Clinical Microbiology, March 2004, p. 981-986, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.981-986.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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