This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Warwick, S.
Right arrow Articles by Millar, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Warwick, S.
Right arrow Articles by Millar, M. R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2004, p. 1402-1408, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1402-1408.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of Quantitative 16S Ribosomal DNA Detection for Diagnosis of Central Vascular Catheter-Associated Bacterial Infection

S. Warwick,1 M. Wilks,1 E. Hennessy,2 J. Powell-Tuck,3 M. Small,3 J. Sharp,3 and M. R. Millar1*

Department of Microbiology, Barts and The London NHS Trust,1 Department of Environmental and Preventive Medicine, Wolfson Institute of Preventive Medicine,2 Department of Human Nutrition, Barts and The London Queen Mary's School of Medicine and Dentistry, London, United Kingdom3

Received 11 September 2003/ Returned for modification 18 November 2003/ Accepted 16 December 2003

Many central vascular catheters (CVCs) are removed unnecessarily because current diagnostic methods for CVC-associated infection are unreliable. A quantitative PCR assay using primers and probe targeted to bacterial 16S ribosomal DNA was used to measure the levels of bacterial DNA in blood samples drawn through the CVC in a population of patients receiving intravenous nutrition. Bacterial DNA concentrations were raised in 16 of 16 blood samples taken during episodes of probable bacterial CVC-associated infection. Bacterial DNA concentrations were raised in 4 of 29 episodes in which bacterial CVC-associated infection was unlikely. The use of this technique has the potential to substantially reduce the unnecessary removal of CVCs.

* Corresponding author. Mailing address: Department of Medical Microbiology, Barts and The London NHS Trust, 37 Ashfield St., London E1 1BB, United Kingdom. Phone: 44 (0)20 7377 7080. Fax: 44 (0)20 7377 7330. E-mail: m.r.millar{at}qmul.ac.uk.

Journal of Clinical Microbiology, April 2004, p. 1402-1408, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1402-1408.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • von Lilienfeld-Toal, M., Lehmann, L. E., Raadts, A. D., Hahn-Ast, C., Orlopp, K. S., Marklein, G., Purr, I., Cook, G., Hoeft, A., Glasmacher, A., Stuber, F. (2009). Utility of a Commercially Available Multiplex Real-Time PCR Assay To Detect Bacterial and Fungal Pathogens in Febrile Neutropenia. J. Clin. Microbiol. 47: 2405-2410 [Abstract] [Full Text]  
  • Bossola, M., Sanguinetti, M., Scribano, D., Zuppi, C., Giungi, S., Luciani, G., Torelli, R., Posteraro, B., Fadda, G., Tazza, L. (2009). Circulating Bacterial-Derived DNA Fragments and Markers of Inflammation in Chronic Hemodialysis Patients. CJASN 4: 379-385 [Abstract] [Full Text]  
  • Zucol, F., Ammann, R. A., Berger, C., Aebi, C., Altwegg, M., Niggli, F. K., Nadal, D. (2006). Real-Time Quantitative Broad-Range PCR Assay for Detection of the 16S rRNA Gene Followed by Sequencing for Species Identification.. J. Clin. Microbiol. 44: 2750-2759 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»