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Journal of Clinical Microbiology, April 2004, p. 1483-1490, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1483-1490.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Analysis of Polymorphic Microsatellite Markers for Typing Penicillium marneffei Isolates

Brent A. Lasker1* and Yuping Ran2

Mycotic Diseases Branch, Division of Bacterial and Mycotic Diseases, National Centers for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 West China Hospital of Sichuan University, Chengdu 610041, China2

Received 15 October 2003/ Returned for modification 18 November 2003/ Accepted 26 November 2003

Penicillium marneffei is an emerging opportunistic dimorphic fungal pathogen that is endemic in Southeast Asia. A typing method based on the analysis of size polymorphisms in microsatellite loci was investigated. Three loci available from the GenBank database were identified to harbor microsatellites. PCR primers flanking the microsatellite repeats were designed with one primer in the set fluorescently labeled. PCR products were then sized by automated capillary electrophoresis. As expected for a haploid fungus, a single band was observed for each microsatellite locus for all isolates. Polymorphic microsatellite marker (PMM) analysis detected a total of 22 different allelic types for 35 isolates of P. marneffei with a high discriminatory power (D = 0.956). Microsatellites I, II, and III detected 14, 10, and 7 alleles, respectively. The reproducibility of length polymorphisms was confirmed by using different DNA preparations from the same isolate or by repeated runs from the same DNA preparation. PMM profiles for eight isolates passaged in vitro for 7 to 8 weeks were identical to the original culture, demonstrating short-term stability and reproducibility. PCR products were not observed for other dimorphic fungi or human DNA. Comparison of allelic frequencies in isolates obtained from China and Thailand identified distinct allele combinations, suggesting the potential geographic isolation of populations. Due to the high discriminatory power, reproducibility, and potential for high throughput, PMM analysis may provide a good typing method for epidemiologic and surveillance investigations of P. marneffei.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, Mailstop G-11, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-3905. Fax: (404) 639-3546. E-mail: blasker{at}cdc.gov.

Journal of Clinical Microbiology, April 2004, p. 1483-1490, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1483-1490.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lasker, B. A. (2006). Nucleotide Sequence-Based Analysis for Determining the Molecular Epidemiology of Penicillium marneffei.. J. Clin. Microbiol. 44: 3145-3153 [Abstract] [Full Text]  
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  • Vanittanakom, N., Cooper, C. R. Jr., Fisher, M. C., Sirisanthana, T. (2006). Penicillium marneffei Infection and Recent Advances in the Epidemiology and Molecular Biology Aspects. Clin. Microbiol. Rev. 19: 95-110 [Abstract] [Full Text]  
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