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Journal of Clinical Microbiology, April 2004, p. 1542-1546, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1542-1546.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of the Automated Phoenix System for Potential Routine Use in the Clinical Microbiology Laboratory

J.-L. Donay,1 D. Mathieu,2 P. Fernandes,1 C. Prégermain,2 P. Bruel,2 A. Wargnier,1 I. Casin,1 F. X. Weill,1 P. H. Lagrange,1 and J. L. Herrmann1*

Service de Microbiologie, Hôpital Saint Louis, Paris,1 Service de Microbiologie, Hôpital Paul Brousse, Villejuif, France2

Received 24 July 2003/ Returned for modification 1 September 2003/ Accepted 7 January 2004

A comparative study was designed to evaluate the identification (ID) and antimicrobial susceptibility testing (AST) performances of the BD Phoenix Automated Microbiology System (Becton Dickinson Diagnostic Systems [BD], Pont de Claix, France). A total of 305 single clinical isolates were collected, and comparisons were made with routine manual methods in use in our microbiology laboratories. The percentages of correct IDs were 93.3, 89.4, 91.8, and 85.7% for enterobacteria, nonfermenting gram-negative bacilli, staphylococci, and streptococci-enterococci, respectively. The median ID time was 3 h, and the median time for AST was 10 h 30 min. AST results showed variable percentages of errors for the different antibiotics. None of the enterobacteria and 0.3% of Pseudomonas aeruginosa isolates showed a very major error (VME). Only one strain of Staphylococcus aureus showed a VME with oxacillin. We demonstrate here the efficiency of the Phoenix system, which can be used for the majority of strains encountered in a university-based laboratory, for ID and AST.

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital Saint Louis, 1 Ave. Claude Vellefaux, 75010 Paris, France. Phone: 33-1-42-49-93-48. Fax: 33-1-42-49-92-00. E-mail: jean-louis.herrmann{at}sls.ap-hop-paris.fr.

Journal of Clinical Microbiology, April 2004, p. 1542-1546, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1542-1546.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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