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Journal of Clinical Microbiology, April 2004, p. 1577-1584, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1577-1584.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Diagnosis of Human Adenoviruses D and E by a Phylogeny-Based Classification Method Using a Partial Hexon Sequence

Yasushi Shimada,1 Toshihide Ariga,2 Yoshitsugu Tagawa,2 Koki Aoki,2 Shigeaki Ohno,2 and Hiroaki Ishiko1*

Research and Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Shimura 3-30-1, Itabashi-ku, Tokyo 174-8555,1 Ophthalmology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo Hokkaido 060-8638, Japan2

Received 7 July 2003/ Returned for modification 10 September 2003/ Accepted 14 December 2003

Human adenoviruses (HAdVs) are the major causes of a variety of acute illnesses. Virus isolation and neutralization tests are usually done to identify the causative virus, but these tests are labor-intensive and time-consuming, and standardized antisera are in limited supply. This study investigated a rapid and reliable method of virus identification based on PCR and phylogenetic analysis. The phylogenetic tree constructed by neighbor joining on the basis of the newly determined partial hexon sequences from 33 prototypes of HAdV-D and -E, along with 11 available prototypes of HAdV-A to -C and -F from GenBank, allowed HAdVs to be grouped into six distinct clusters. These clusters correspond closely to the six newly designated species, HAdV-A to -F. The partial hexon sequences of 57 isolates from patients with acute conjunctivitis obtained over 20 years plus those of 44 prototype strains were analyzed. Each isolate formed a monophyletic cluster along with its respective prototype strain, allowing serotype identification. Partial-hexon-based classification appears to be an effective tool for studying the molecular epidemiology of HAdVs.

* Corresponding author. Mailing address: Research and Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Shimura 3-30-1, Itabashi-ku, Tokyo 174-8555, Japan. Phone: 81-3-5994-2431. Fax: 81-3-5994-2972. E-mail: Ishiko{at}m1.mbcL.co.jp.

Journal of Clinical Microbiology, April 2004, p. 1577-1584, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1577-1584.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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