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Journal of Clinical Microbiology, April 2004, p. 1605-1608, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1605-1608.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Chromatography Paper Strip Method for Collection, Transportation, and Storage of Rotavirus RNA in Stool Samples

Mustafizur Rahman,1,2 Truus Goegebuer,1 Karolien De Leener,1 Piet Maes,1 Jelle Matthijnssens,1 Goutam Podder,2 Tasnim Azim,2 and Marc Van Ranst1,3*

Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven,1 Department of Laboratory Medicine, University Hospital Gasthuisberg, Leuven, Belgium,3 Laboratory of Virology, ICDDR,B: Centre for Health and Population Research, Dhaka, Bangladesh2

Received 21 November 2002/ Returned for modification 3 March 2003/ Accepted 9 December 2003

We developed a novel method that uses sodium dodecyl sulfate-EDTA-treated chromatography paper strips to collect unconcentrated fresh stool samples. After the paper strips were stored for 4 months at room temperature, rotavirus RNA could be successfully amplified by using reverse transcriptase PCR. The use of filter paper strips as a specimen support allows (self-)collection of stool samples by untrained persons. Diarrheal stool samples from remote areas can be stored and transported to a central diagnostic laboratory without the need for freezers or special shipping conditions. This convenient and inexpensive rotavirus sample collection system can be of use in epidemiological surveillance studies and vaccine trials.

* Corresponding author. Mailing address: Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Minderbroedersstraat 10, BE-3000 Leuven, Belgium. Phone: 32-16-347908. Fax: 32-16-332131. E-mail: marc.vanranst{at}uz.kuleuven.ac.be.

Journal of Clinical Microbiology, April 2004, p. 1605-1608, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1605-1608.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Copyright © 2004 by the American Society for Microbiology. All rights reserved.