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Journal of Clinical Microbiology, April 2004, p. 1614-1619, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1614-1619.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Simple Algorithm for Identification of Bordetella pertussis Pertactin Gene Variants

Gaëtan Muyldermans,1 Denis Piérard,1* Nathalie Hoebrekx,1 Reza Advani,2 Shirley Van Amersfoorth,3 Iris De Schutter,4 Oriane Soetens,1 Leo Eeckhout,1 Anne Malfroot,4 and Sabine Lauwers1

Belgian Reference Laboratory for Pertussis, Department of Microbiology,1 Department of Pediatrics, Academisch Ziekenhuis Vrije Universiteit Brussel, Brussels, Belgium,4 Swedish Institute for Infectious Disease Control, Solna, Sweden,2 Research Laboratory for Infectious Diseases, National Institute for Public Health and Environment (RIVM), Bilthoven, The Netherlands3

Received 18 July 2003/ Returned for modification 5 September 2003/ Accepted 12 January 2004

Studies performed in several countries have demonstrated the recent emergence and subsequent dominance of circulating Bordetella pertussis strains harboring pertactin and pertussis toxin variants not included in pertussis vaccines. Determination of the pertactin gene variants is commonly performed using a time-consuming and expensive sequence analysis. We developed a simple and reliable pertactin typing algorithm suitable for large-scale screening. The assay correctly identified all pertactin alleles in representative strains. The typing of 231 clinical strains of B. pertussis routinely isolated in Belgium showed that this algorithm was adequate to identify less-frequent prn types like prn9 and prn11.

* Corresponding author. Mailing address: Department of Microbiology, Academisch Ziekenhuis Vrije Universiteit Brussel, Laarbeeklaan 101, B-1090 Brussels, Belgium. Phone: 32 2 477 50 02. Fax: 32 2 477 50 15. E-mail: denis.pierard{at}az.vub.ac.be.

Journal of Clinical Microbiology, April 2004, p. 1614-1619, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1614-1619.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2004 by the American Society for Microbiology. All rights reserved.