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Journal of Clinical Microbiology, April 2004, p. 1641-1647, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1641-1647.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of ompA Genotypes by Sequence Analysis of DNA from All Detected Cases of Chlamydia trachomatis Infections during 1 Year of Contact Tracing in a Swedish County

Section of Clinical Bacteriology, Department of Medical Sciences, University of Uppsala, Uppsala,¹ Department of Clinical Microbiology, Central Hospital, Växjö,² Department of Communicable Disease Control and Prevention, Central Hospital, Karlstad,⁴ Section of Sexually Transmitted Infections, Department of Clinical Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden³

Received 4 July 2003/ Returned for modification 27 October 2003/ Accepted 18 December 2003

In this study we aimed to characterize the ompA gene by sequencing DNA from all detected cases of Chlamydia trachomatis infection in a Swedish county during 2001, in order to improve the efficiency of contact tracing. Approximately 990 bp of the ompA gene was amplified, and sequence analysis was achieved in 678 (94%) of 725 C. trachomatis-positive cases in this unselected population. The most prevalent genotype was serotype E (39%), followed by F (21%), G (11%), D (9%), K (9%), J (7%), H (2%), B (1%), and Ia (1%). Serotype E was found in five genotype variants, with the reference sequence comprising 96% of all E cases. Serotype D was the most variable, and of seven sequence variants, three were identified as recombinants with serotype E. Altogether 29 genetic variants were detected, and mutations and recombination events are discussed. Clinical manifestations were not associated with genotypes. Sequence variation was linked to sexual networks identified by contact tracing and improved epidemiological knowledge but was of limited benefit.

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