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Journal of Clinical Microbiology, April 2004, p. 1716-1718, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1716-1718.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Quality Control Limits for Voriconazole Disk Susceptibility Tests on Mueller-Hinton Agar with Glucose and Methylene Blue

M. A. Pfaller,1* A. Barry,2 J. Bille,3 S. Brown,2 D. Ellis,4 J. F. Meis,5 R. Rennie,6 M. Rinaldi,7 T. Rogers,8 and M. Traczewski2

University of Iowa, Iowa City, Iowa,1 The Clinical Microbiology Institute, Wilsonville, Oregon,2 Institute of Microbiology, University Hospital, Lausanne, Switzerland,3 Women and Children's Hospital, North Adelaide, South Australia, Australia,4 Department of Medical Microbiology and Infectious Diseases, Nijmegen, The Netherlands,5 University of Alberta Hospital, Edmonton, Alberta, Canada,6 University of Texas Health Science Center, San Antonio, Texas,7 Imperial College, Hammersmith, London, England8

Received 21 October 2003/ Returned for modification 28 November 2003/ Accepted 14 December 2003

An international collaborative study was performed in order to propose quality control limits for voriconazole disk diffusion tests on Mueller-Hinton agar with 2% glucose and 0.5 µg of methylene blue per ml. The supplement may be added to the agar before autoclaving, or Mueller-Hinton agar plates may be flooded with a glucose-methylene blue solution. Replicate tests on both types of agar plates with 1-µg voriconazole disks generated data to propose zone size limits for tests of Candida parapsilosis ATCC 22019 (28 to 37 mm), Candida albicans ATCC 90028 (31 to 42 mm), and Candida krusei ATCC 6258 (16 to 25 mm). Candida tropicalis ATCC 750 was not useful for this purpose.

* Corresponding author. Mailing address: Medical Microbiology Division, C606 GH, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242. Phone: (319) 384-9566. Fax: (319) 356-4916. E-mail: michael-pfaller{at}uiowa.edu.

Journal of Clinical Microbiology, April 2004, p. 1716-1718, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1716-1718.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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