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Journal of Clinical Microbiology, April 2004, p. 1753-1755, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1753-1755.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

First International Quality Assurance Study on the Rapid Detection of Viral Agents of Bioterrorism

Matthias Niedrig,1 Herbert Schmitz,2 Stephan Becker,3 Stephan Günther,2 Jan ter Meulen,3,{dagger} Herman Meyer,4 Heinz Ellerbrok,1 Andreas Nitsche,1 Hans R. Gelderblom,1 and Christian Drosten2*

Robert Koch-Institute, Berlin,1 Bernhard-Nocht-Institute, Hamburg,2 Institute of Virology, Marburg,3 Institute of Microbiology of the Bundeswehr, München, Germany4

Received 15 August 2003/ Returned for modification 12 October 2003/ Accepted 31 December 2003

We have conducted an international quality assurance study of filovirus, Lassa virus, and orthopox virus PCR with 24 participants. Of the participating laboratories, 45.8 and 66.7% detected virus in all plasma samples, which contained >=5,000 and >=100,000 copies per ml, respectively. Sensitivity levels were not significantly different between viruses. False-negative results were attributable to a lack of sensitivity.

* Corresponding author. Mailing address: Bernhard-Nocht-Institute for Tropical Medicine, Bernhard-Nocht Str. 74, 20359 Hamburg, Germany. Phone: 49-40-42818-421. Fax: 49-40-42818-378. E-mail: drosten{at}bni-hamburg.de.

{dagger} Present address: Crucell B.V., Leiden, The Netherlands.

Journal of Clinical Microbiology, April 2004, p. 1753-1755, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1753-1755.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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