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Reactivation of Porcine Cytomegalovirus through Allogeneic Stimulation

Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108,¹ Nextran, Inc., Princeton, New Jersey 08540²

Received 3 June 2003/ Returned for modification 19 August 2003/ Accepted 4 November 2003

Reactivation of latent porcine cytomegalovirus after coculture of peripheral blood mononuclear cells (PBMCs) from pigs with different genetic backgrounds was investigated. Nine of 10 allogeneic coculture pairs were PCR (DNA) positive, whereas 7 coculture pairs had porcine cytomegalovirus (PCMV) RNA, an indication of virus replication. The cell subpopulations harboring PCMV were monocytes and CD8⁺ T cells.

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