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Journal of Clinical Microbiology, April 2004, p. 1777-1778, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1777-1778.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Nested PCR Assay for Detection of Leishmania donovani in Slit Aspirates from Post-Kala-Azar Dermal Leishmaniasis Lesions

Gannavaram Sreenivas,1 N. A. Ansari,1 Joginder Kataria,2 and Poonam Salotra1*

Institute of Pathology (ICMR),1 Department of Dermatology, Safdarjung Hospital Campus, New Delhi, India2

Received 2 September 2003/ Returned for modification 2 November 2003/ Accepted 16 January 2004

A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner.

* Corresponding author. Mailing address: Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, India. Phone: 91 11 2616 6124. Fax: 91 11 2619 8401. E-mail: salotra{at}vsnl.com.

Journal of Clinical Microbiology, April 2004, p. 1777-1778, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1777-1778.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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