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Journal of Clinical Microbiology, April 2004, p. 1787-1789, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1787-1789.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multiplex PCR for Diagnosis of Enteric Infections Associated with Diarrheagenic Escherichia coli

Roberto Vidal,1* Maricel Vidal,1 Rossana Lagos,2 Myron Levine,3 and Valeria Prado1

Programa de Microbiologia, Institode Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile,1 Centro para Vacunas en Desarrollo, Santiago, Chile,2 Center for Vaccine Development, University of Maryland, Baltimore, Maryland3

Received 30 July 2003/ Returned for modification 22 September 2003/ Accepted 6 January 2004

A multiplex PCR for detection of three categories of diarrheagenic Escherichia coli was developed. With this method, enterohemorrhagic E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli were identified in fecal samples from patients with hemorrhagic colitis, watery diarrhea, or hemolytic-uremic syndrome and from food-borne outbreaks.

* Corresponding author. Mailing address: Programa de Microbiología, Facultad de Medicina, Universidad de Chile, Av. Independencia 1027, Santiago, Chile. Phone: 56-2-678 6646. Fax: 56-2-735 5855. E-mail: rvidal{at}machi.med.uchile.cl.

Journal of Clinical Microbiology, April 2004, p. 1787-1789, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1787-1789.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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