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Journal of Clinical Microbiology, April 2004, p. 1832-1836, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1832-1836.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genotyping and Antifungal Susceptibility Profile of Dipodascus capitatus Isolates Causing Disseminated Infection in Seven Hematological Patients of a Tertiary Hospital

Ignacio Gadea,1 Manuel Cuenca-Estrella,2* Elena Prieto,3 Teresa M. Diaz-Guerra,2 Jose I. Garcia-Cia,1 Emilia Mellado,2 Jose F. Tomas,3 and Juan L. Rodriguez-Tudela2

Departamento de Microbiología,1 Departamento de Hematología, Fundación Jiménez Díaz,3 Unidad de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain2

Received 14 November 2003/ Returned for modification 9 December 2003/ Accepted 18 December 2003

Seven cases of disseminated infection due to Dipodascus capitatus are reported. Infections occurred in a hematological unit of a tertiary hospital during a period of 5 years. Five cases were refractory to antifungal therapy. Antifungal susceptibility testing of seven isolates was performed, and strains were typed by PCR fingerprinting with the core sequence of phage M13 and by random amplification of polymorphic DNA with two primers, Ap12h and W-80A. A very short range of MICs of each antifungal agent was observed. The MICs of amphotericin B ranged between 0.50 and 2 µg/ml. Strains were susceptible in vitro to flucytosine and susceptible (dose-dependent) to fluconazole and itraconazole. Voriconazole exhibited an activity in vitro comparable to that of itraconazole. Typing techniques allowed seven additional isolates of D. capitatus neither geographically nor temporally related to be classified into two different genomic patterns. The genomic type of the seven strains from the hematological unit was identical regardless of typing technique utilized. It would indicate that the seven cases of disseminated infection could be related epidemiologically.

* Corresponding author. Mailing address: Unidad de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220 Majadahonda (Madrid), Spain. Phone: 34-91-5097961. Fax: 34-91-5097966. E-mail: mcuenca-estrella{at}isciii.es.

Journal of Clinical Microbiology, April 2004, p. 1832-1836, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1832-1836.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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