This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oya, C.
Right arrow Articles by Hondo, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oya, C.
Right arrow Articles by Hondo, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2004, p. 1869-1874, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.1869-1874.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Specific Detection and Identification of Herpes B Virus by a PCR-Microplate Hybridization Assay

Chika Oya,1,2 Yoshitsugu Ochiai,1 Yojiro Taniuchi,1,{dagger} Takashi Takano,1,{ddagger} Fukiko Ueda,1 Yasuhiro Yoshikawa,2 and Ryo Hondo1*

Department of Veterinary Public Health, Nippon Veterinary and Animal Science University, Musashino, Tokyo 180-8602,1 Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan2

Received 2 September 2003/ Returned for modification 9 November 2003/ Accepted 1 February 2004

Herpes B virus DNA was specifically amplified by PCR, targeting the regions that did not cross-react with herpes simplex virus (HSV). The amplified products, which were shown to be highly genetic polymorphisms among herpes B virus isolates, were identified by microplate hybridization with probes generated by PCR. The products immobilized in microplate wells were hybridized with the biotin-labeled probes derived from the SMHV strain of herpes B virus. The amplified products derived from the SMHV and E2490 strains of herpes B virus were identified by microplate hybridization. PCR products amplified from the trigeminal ganglia of seropositive cynomolgus macaques were identified as herpes B virus DNA. The utility of the PCR-microplate hybridization assay for genetic detection and identification of the polymorphic region of herpes B virus was determined.

* Corresponding author. Mailing address: Department of Veterinary Public Health, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan, Musashino, Tokyo 180-8602, Japan. Phone: 81-422-31-4151, ext. 282. Fax: 81-422-30-7531. E-mail: nvau-vph{at}interlink.or.jp.

{dagger} Present address: Department of Molecular Immunology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.

{ddagger} Present address: Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.

Journal of Clinical Microbiology, May 2004, p. 1869-1874, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.1869-1874.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Oya, C, Ochiai, Y, Taniuchi, Y, Takano, T, Fujima, A, Ueda, F, Hondo, R, Yoshikawa, Y (2008). Prevalence of herpes B virus genome in the trigeminal ganglia of seropositive cynomolgus macaques. Lab Anim 42: 99-103 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»