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Journal of Clinical Microbiology, May 2004, p. 1962-1964, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.1962-1964.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Specific Identification of Staphylococcus aureus by Staphychrom II, a Rapid Chromogenic Staphylocoagulase Test

Hôpital de Bellevue, F-42055 Saint Etienne,¹ CNR des Staphylocoques, INSERM E-0230, Faculté de Médecine Laennec, F-69372 Lyon cedex 8,² Hôpital Debrousse, Hospices Civils de Lyon, F-69322 Lyon cedex 5, France³

Received 29 October 2003/ Returned for modification 18 December 2003/ Accepted 31 January 2004

We compared the performance of Staphychrom II (International Microbio, Signes, France), a rapid (2-h) chromogenic staphylocoagulase test that uses human prothrombin and protease inhibitors, with those of the reference tube coagulase test (TCT) and the latex agglutination test (LAT) Slidex Staph Plus for the rapid identification of S. aureus. Prospective evaluation with 293 fresh clinical isolates yielded sensitivities, specificities, and predictive and negative predictive values of 98.1, 100, 100, and 95.1%, respectively, for the Staphychrom II test; 98.6, 98.7, 99.6, and 96.3%, respectively, for LAT; and 97.6, 98.7, 99.5, and 93.9%, respectively, for TCT. The perfect specificity of the Staphychrom II test was confirmed by testing 193 collection strains selected because of their potential testing pitfalls. The Staphychrom II test was positive for 90% of the 215 S. aureus strains tested after only 1 h of incubation. The Staphychrom II test was as sensitive as the reference TCT and was 100% specific.

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