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Journal of Clinical Microbiology, May 2004, p. 1982-1985, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.1982-1985.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Amplification of Coccidioidal DNA in Clinical Specimens by PCR

Suzanne M. Johnson,* Keira A. Simmons, and Demosthenes Pappagianis

Department of Medical Microbiology and Immunology, School of Medicine, University of California—Davis, Davis, California 95616

Received 28 October 2003/ Returned for modification 7 January 2004/ Accepted 9 February 2004

Coccidioides DNA was amplified from serum by a PCR using coccidioid-specific primers. A 239-bp product was visualized when 10 fg of exogenous coccidioidal DNA was subjected to amplification. This product was demonstrated in some human and mouse sera prior to the detection of coccidioidal antibodies.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, School of Medicine, University of California—Davis, Davis, CA 95616. Phone: (530) 752-7214. Fax: (530) 752-8692. E-mail: smjohnson{at}ucdavis.edu.

Journal of Clinical Microbiology, May 2004, p. 1982-1985, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.1982-1985.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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