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Journal of Clinical Microbiology, May 2004, p. 2101-2107, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2101-2107.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Candida in Concentrated Oral Rinse Cultures by Real-Time PCR

P. Lewis White,1 David W. Williams,2* Tomoari Kuriyama,2 Shamim A. Samad,1 Michael A. O. Lewis,2 and Rosemary A. Barnes1

Department of Medical Microbiology and PHLS, University Hospital of Wales,1 Department of Oral Surgery, Medicine and Pathology, University of Wales College of Medicine, Cardiff, United Kingdom2

Received 24 September 2003/ Returned for modification 15 October 2003/ Accepted 26 November 2003

The incidence of oral candidosis has increased in recent years, largely as a result of the emergence of human immunodeficiency virus infection and the more widespread use of immunosuppressive chemotherapy. This development has been associated with a need for more reliable methods for the detection of Candida. The present study assessed the performance of a real-time PCR and two block-based PCRs for the detection of Candida in 193 concentrated oral rinse culture (CRC) specimens. A total of 102 CRC specimens were positive by culture for Candida; and 96, 90, and 75 of these were also positive by real-time, N18-specific, and internal transcribed spacer (ITS)-specific PCRs, respectively. The five false-negative results by the real-time PCR were all non-Candida albicans positive by culture. Of the 91 culture-negative CRC specimens, 20, 41, and 44 were positive by the real-time PCR and the N18- and ITS-specific PCRs, respectively. All three PCRs detected fungal DNA in 8 culture-negative CRC specimens, with a further 30 being positive by two of the three PCRs. A total of 32 CRC specimens were Candida free by all methods. In summary, a real-time PCR that provides a sensitive, specific, and rapid alternative technique for detection of Candida in the mouth is described.

* Corresponding author. Mailing address: Department of Oral Surgery, Medicine and Pathology, University of Wales College of Medicine, Cardiff, United Kingdom. Phone and fax: (44) 2920742442. E-mail: williamsdd{at}cf.ac.uk.

Journal of Clinical Microbiology, May 2004, p. 2101-2107, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2101-2107.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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