This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klaassen, C. H. W. Articles by Thunnissen, F. B. J. M. Search for Related Content PubMed PubMed Citation Articles by Klaassen, C. H. W. Articles by Thunnissen, F. B. J. M.

DNA Microarray Format for Detection and Subtyping of Human Papillomavirus

Departments of Pathology,¹ Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen,² Dot Diagnostics, Beuningen, The Netherlands³

Received 17 November 2003/ Accepted 30 December 2003

A new human papillomavirus (HPV) assay using high-density DNA microarrays is described. An HPV DNA fragment from the 3' end of the E1 gene was amplified and digoxigenin labeled by PCR, and the resulting amplicons were hybridized onto type-specific oligonucleotides immobilized on high-density DNA microarrays. For detection, a simple immunohistochemical staining procedure was used with a substrate that has both colorimetric and fluorescent properties. This detection chemistry enables the rapid identification of reactive spots by regular light microscopy and semiquantification by laser scanning. Both single and multiple HPV infections are recognized by this assay, and the corresponding HPV types are easily identified. With this assay, 53 mucosal HPV types were detected and identified. A total of 45 HPV types were identified by a single type-specific probe, whereas the remaining 8 mucosal HPV types could be identified by a specific combination of probes. The simple assay format allows usage of this assay without expensive equipment, making it accessible to all diagnostic laboratories with PCR facilities.

This article has been cited by other articles:

• Brukner, I., El-Ramahi, R., Gorska-Flipot, I., Krajinovic, M., Labuda, D. (2007). An in vitro selection scheme for oligonucleotide probes to discriminate between closely related DNA sequences. Nucleic Acids Res 35: e66-e66 [Abstract] [Full Text]  
• Jiang, H.-L., Zhu, H.-H., Zhou, L.-F., Chen, F., Chen, Z. (2006). Genotyping of human papillomavirus in cervical lesions by L1 consensus PCR and the Luminex xMAP system. J Med Microbiol 55: 715-720 [Abstract] [Full Text]