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Journal of Clinical Microbiology, May 2004, p. 2203-2206, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2203-2206.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of ß-Lactamase Inhibitors in Disk Tests To Detect Plasmid-Mediated AmpC ß-Lactamases

Jennifer A. Black,1 Kenneth S. Thomson,1 and Johann D. D. Pitout2*

Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska,1 Division of Microbiology, Calgary Laboratory Services, and Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada2

Received 26 November 2003/ Returned for modification 7 January 2004/ Accepted 20 January 2004

Seeking a simple disk test for detection of organisms producing plasmid-mediated AmpC ß-lactamases, we evaluated the diagnostic utility of the ß-lactamase inhibitors 48-1220 (Ro 48-1220) and LN-2-128. Using NCCLS disk methodology, inhibition zone diameters were determined for five ß-lactam antibiotics tested alone and in combination with 20 µg of either 48-1220 or LN-2-128. Using an increase of >=4 mm in zone diameter in the presence of an inhibitor as a positive test, cefotetan with LN-2-128 and 48-1220 was adequate for the detection of organisms producing plasmid-mediated AmpCs (specificity of 90% and sensitivity of 100%).

* Corresponding author. Mailing address: Division of Microbiology, Calgary Laboratory Services and Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada T3C 0J9. Phone: (403) 209-5309. Fax: (403) 209-5347. E-mail: johann.pitout{at}cls.ab.ca.

Journal of Clinical Microbiology, May 2004, p. 2203-2206, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2203-2206.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jacoby, G. A. (2009). AmpC {beta}-Lactamases. Clin. Microbiol. Rev. 22: 161-182 [Abstract] [Full Text]  
  • Black, J. A., Thomson, K. S., Buynak, J. D., Pitout, J. D. D. (2005). Evaluation of {beta}-Lactamase Inhibitors in Disk Tests for Detection of Plasmid-Mediated AmpC {beta}-Lactamases in Well-Characterized Clinical Strains of Klebsiella spp.. J. Clin. Microbiol. 43: 4168-4171 [Abstract] [Full Text]  
  • Black, J. A., Moland, E. S., Thomson, K. S. (2005). AmpC Disk Test for Detection of Plasmid-Mediated AmpC {beta}-Lactamases in Enterobacteriaceae Lacking Chromosomal AmpC {beta}-Lactamases. J. Clin. Microbiol. 43: 3110-3113 [Abstract] [Full Text]  
  • Yagi, T., Wachino, J.-i., Kurokawa, H., Suzuki, S., Yamane, K., Doi, Y., Shibata, N., Kato, H., Shibayama, K., Arakawa, Y. (2005). Practical Methods Using Boronic Acid Compounds for Identification of Class C {beta}-Lactamase-Producing Klebsiella pneumoniae and Escherichia coli. J. Clin. Microbiol. 43: 2551-2558 [Abstract] [Full Text]  


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