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Journal of Clinical Microbiology, May 2004, p. 2218-2220, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2218-2220.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development and Evaluation of a New Commercial Test Allowing the Simultaneous Detection of Noroviruses and Sapoviruses by Reverse Transcription-PCR and Microplate Hybridization

F. Bon,1,2 H. Giraudon,1,2 C. Sancey,3 C. Barranger,3 M. Joannes,3 P. Pothier,1,2 and E. Kohli1,2*

Laboratoire de Virologie, Reference Laboratory for Enteric Viruses, Centre Hospitalier Universitaire,1 Microbiologie Médicale et Moléculaire, UFRS Médecine et Pharmacie, Dijon,2 Laboratoire Argene-Biosoft, Varilhes, France3

Received 1 April 2003/ Returned for modification 24 April 2003/ Accepted 20 October 2003

This work describes the design and initial evaluation of a commercial test allowing the detection of noroviruses and sapoviruses by reverse transcription-PCR (RT-PCR) in a single tube followed by microplate hybridization, as well as the detection of PCR inhibitors. The test was shown to be broadly reactive (except for Melksham-like strains), sensitive, and specific and thus should be useful for calicivirus detection in clinical practice.

* Corresponding author. Mailing address: Microbiologie Médicale et Moléculaire, Facultés de Médecine et Pharmacie, 7 Bd. Jeanne d'Arc, 21033 Dijon, France. Phone: 33 3 80 29 34 37. Fax: 33 3 80 29 36 04. E-mail: evelyne.kohli{at}chu-dijon.fr.

Journal of Clinical Microbiology, May 2004, p. 2218-2220, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2218-2220.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Copyright © 2004 by the American Society for Microbiology. All rights reserved.