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Journal of Clinical Microbiology, May 2004, p. 2234-2238, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2234-2238.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Fingerprinting of Mycobacterium bovis subsp. caprae Isolates from Central Europe

Wilfried Erler,1 Gerald Martin,1 Konrad Sachse,1* Ludmila Naumann,2,3 Dagmar Kahlau,3 Jörg Beer,4 Milan Bartos,5 György Nagy,6 Zeljko Cvetnic,7 Manca Zolnir-Dovc,8 and Ivo Pavlik5

Federal Research Centre for Virus Diseases of Animals (BFAV), Jena,1 Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg,2 Bavarian State Office for Health and Food Safety, Oberschleissheim,3 Institute of Medical Microbiology, University of Leipzig, Leipzig,4 Germany; Veterinary Research Institute, Brno, Czech Republic,5 Central Veterinary Institute, Budapest, Hungary,6 Croatian Veterinary Institute, Zagreb, Croatia,7 University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia8

Received 6 June 2003/ Returned for modification 19 August 2003/ Accepted 7 January 2004

To study the dissemination of Mycobacterium bovis subsp. caprae, 79 European isolates from cattle, humans, and other hosts were examined by spoligotyping and IS6110 restriction fragment length polymorphism (RFLP) analysis. Among a total of 11 different spoligotypes identified, type C1 proved to be predominant (n = 62). Five of the spoligotypes are described for the first time. A total of 43 different RFLP types were identified, thus allowing further differentiation for epidemiological tracking. Isolates from a series of outbreaks in one village proved to be of the same spoligotype and of identical or closely related RFLP types.

* Corresponding author. Mailing address: Bundesforschungsanstalt für Viruskrankheiten der Tiere (BFAV), Institut für Bakterielle Infektionen und Zoonosen, Naumburger Str. 96a, 07743 Jena, Germany. Phone: 49 3641 8040. Fax: 49 3641 804228. E-mail: k.sachse{at}jena.bfav.de.

Journal of Clinical Microbiology, May 2004, p. 2234-2238, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2234-2238.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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