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Journal of Clinical Microbiology, May 2004, p. 2241-2246, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2241-2246.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Point Mutations in the gyrA Gene of Helicobacter pylori Conferring Resistance to Ciprofloxacin by a Fluorescence Resonance Energy Transfer-Based Real-Time PCR Approach

Erik Glocker and Manfred Kist*

Department of Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital Freiburg, 79104 Freiburg, Germany

Received 24 September 2003/ Returned for modification 19 November 2003/ Accepted 20 January 2004

As a result of the increasing resistance of Helicobacter pylori against first-line antibiotics, other drugs, such as quinolones, will be needed for eradication therapy in the future. We developed a real-time PCR to detect mutations in the gyrA gene associated with ciprofloxacin resistance of H. pylori, thereby contributing to the selection of patients who could be treated by ciprofloxacin-based therapy.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Freiburg, Hermann-Herder-Str. 11, D-79104 Freiburg, Germany. Phone: 49-761-203-6590. Fax: 49-761-203-6562. E-mail: kistman{at}ukl.uni-freiburg.de.

Journal of Clinical Microbiology, May 2004, p. 2241-2246, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2241-2246.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chisholm, S. A., Owen, R. J. (2009). Frequency and molecular characteristics of ciprofloxacin- and rifampicin-resistant Helicobacter pylori from gastric infections in the UK. J Med Microbiol 58: 1322-1328 [Abstract] [Full Text]  
  • Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  
  • Nishizawa, T., Suzuki, H., Umezawa, A., Muraoka, H., Iwasaki, E., Masaoka, T., Kobayashi, I., Hibi, T. (2007). Rapid Detection of Point Mutations Conferring Resistance to Fluoroquinolone in gyrA of Helicobacter pylori by Allele-Specific PCR. J. Clin. Microbiol. 45: 303-305 [Abstract] [Full Text]  
  • Glocker, E., Berning, M., Gerrits, M. M., Kusters, J. G., Kist, M. (2005). Real-Time PCR Screening for 16S rRNA Mutations Associated with Resistance to Tetracycline in Helicobacter pylori. Antimicrob. Agents Chemother. 49: 3166-3170 [Abstract] [Full Text]  


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