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Journal of Clinical Microbiology, May 2004, p. 2247-2248, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2247-2248.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of Flow Cytometric and Alamar Blue Tests with the Proportional Method for Testing Susceptibility of Mycobacterium tuberculosis to Rifampin and Isoniazid

Roberto S. Reis,1 Ivan Neves Jr.,1 Sergio L. S. Lourenço,1 Leila S. Fonseca,2 and Maria Cristina S. Lourenço1*

Oswaldo Cruz Fundation (FIOCRUZ),1 Microbiology Institute, Rio de Janeiro Federal University (UFRJ), Rio de Janeiro, Brazil2

Received 27 October 2003/ Returned for modification 4 December 2003/ Accepted 23 January 2004

The performance of flow cytometry and the microplate Alamar Blue assay in determining susceptibility of Mycobacterium tuberculosis was assessed by testing 150 Brazilian isolates. The overall agreement was 97.3 and 98% for isoniazid and 94.7 and 100% for rifampin by flow cytometry and MABA, respectively. This study was entirely done in a developing country.

* Corresponding author. Mailing address: Fundação Oswaldo Cruz, Instituto de Pesquisa Clínica Evandro Chagas, Serviço de Bacteriologia, Av. Brasil 4365, Rio de Janeiro, RJ 21045-900, Brazil. Phone: 55-21-25984260. Fax: 55-21-22904532. E-mail: lourenco{at}ipec.fiocruz.br.

Journal of Clinical Microbiology, May 2004, p. 2247-2248, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2247-2248.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Copyright © 2004 by the American Society for Microbiology. All rights reserved.