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Journal of Clinical Microbiology, May 2004, p. 2252-2254, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2252-2254.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Collaborative Study of Antibiotic Medium 3 and Flow Cytometry for Identification of Amphotericin B-Resistant Candida Isolates

Vishnu Chaturvedi,1,2* Rama Ramani,1 and John H. Rex3,{dagger}

Mycology Laboratory, Wadsworth Center, New York State Department of Health,1 Department of Biomedical Sciences, School of Public Health, State University of New York at Albany, Albany, New York,2 University of Texas Medical School, Houston, Texas3

Received 29 October 2003/ Returned for modification 4 December 2003/ Accepted 31 January 2004

Center 1 used the National Committee for Clinical Laboratory Standards M27-A2 method and antibiotic medium 3 (AM3) test to determine amphotericin B resistance in 5 of 30 Candida isolates. These isolates were tested at center 2 by AM3 test and flow cytometry (FC). The agreements (C1-C2) were 90% for AM3 test and FC and 73% for the AM3 tests.

* Corresponding author. Mailing address: Mycology Laboratory, Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY 12208-2002. Phone: (518) 474-4177. Fax: (518) 486-7811. E-mail: vishnu{at}wadsworth.org.

{dagger} Present address: AstraZeneca, Alderley Park, Macclesfield, Cheshire SK10 4TF, United Kingdom.

Journal of Clinical Microbiology, May 2004, p. 2252-2254, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2252-2254.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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