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Journal of Clinical Microbiology, May 2004, p. 2255-2257, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2255-2257.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Quantitative Microbiological Study of Subgingival Plaque by Real-Time PCR Shows Correlation between Levels of Tannerella forsythensis and Fusobacterium spp.

Nao Suzuki,1,2 Akihiro Yoshida,2,3 Toshiyuki Saito,2 Miki Kawada,2 and Yoshio Nakano2*

Department of Oral Bacteriology, Ohu University School of Dentistry, Tomitamachi, Koriyama 963-8611,1 Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Higashi-ku, Fukuoka 812-8582,2 Department of Preventive Dentistry, Kyushu Dental College, Kokurakita-ku, Kitakyushu 803-8580, Japan3

Received 2 November 2003/ Returned for modification 15 December 2003/ Accepted 11 February 2004

A TaqMan-based real-time PCR assay was established to quantify the periodontopathic bacteria Tannerella forsythensis and Fusobacterium spp. With this assay, the prevalence and proportion of these bacteria in clinical specimens were evaluated. Our preliminary results suggest a positive colocalization of T. forsythensis and Fusobacterium spp. in periodontal pockets.

* Corresponding author. Mailing address: Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan. Phone: 81-92-642-6423. Fax: 81-92-642-6354. E-mail: yosh{at}dent.kyushu-u.ac.jp.

Journal of Clinical Microbiology, May 2004, p. 2255-2257, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2255-2257.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kurata, H., Awano, S., Yoshida, A., Ansai, T., Takehara, T. (2008). The prevalence of periodontopathogenic bacteria in saliva is linked to periodontal health status and oral malodour. J Med Microbiol 57: 636-642 [Abstract] [Full Text]  
  • Suzuki, N., Yoshida, A., Nakano, Y. (2005). Quantitative Analysis of Multi-Species Oral Biofilms by TaqMan Real-Time PCR. Clin Med Res 3: 176-185 [Abstract] [Full Text]  
  • Nagashima, S., Yoshida, A., Suzuki, N., Ansai, T., Takehara, T. (2005). Use of the Genomic Subtractive Hybridization Technique To Develop a Real-Time PCR Assay for Quantitative Detection of Prevotella spp. in Oral Biofilm Samples. J. Clin. Microbiol. 43: 2948-2951 [Abstract] [Full Text]  
  • Yoshida, A., Nagashima, S., Ansai, T., Tachibana, M., Kato, H., Watari, H., Notomi, T., Takehara, T. (2005). Loop-Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. J. Clin. Microbiol. 43: 2418-2424 [Abstract] [Full Text]  


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