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Journal of Clinical Microbiology, May 2004, p. 2264-2267, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2264-2267.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Presence of Bacterial Phage-Like DNA Sequences in Commercial Taq DNA Polymerase Reagents

Tamara Newsome,1 Bing-Jie Li,1 Nianxiang Zou,1 and Shyh-Ching Lo2*

Armed Forces Institute of Pathology,2 Department of Infectious and Parasitic Disease Pathology, American Registry of Pathology, Washington, D.C.1

Received 26 November 2003/ Returned for modification 27 January 2004/ Accepted 17 February 2004

Many studies have reported the presence of bacterial DNA contamination in commercial Taq DNA polymerase reagents. This is the first report of the presence of phage-like DNA sequences in certain commercial Taq DNA polymerase reagents. Precautions are needed when using amplification reagents with exogenous DNAs.

* Corresponding author. Mailing address: Department of Infectious and Parasitic Disease Pathology, Armed Forces Institute of Pathology, Bldg. 54, Room 4091, 14th & Alaska Ave., NW, Washington, DC 20306-6000. Phone: (202) 782-1870. Fax: (202) 782-9160. E-mail: LOS{at}afip.osd.mil.

Journal of Clinical Microbiology, May 2004, p. 2264-2267, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2264-2267.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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