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Journal of Clinical Microbiology, May 2004, p. 2275-2278, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2275-2278.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Candida albicans mRNA in Archival Histopathology Samples by Reverse Transcription-PCR

Kyle T. Beggs,1 Ann R. Holmes,1 Richard D. Cannon,1* and Alison M. Rich2

Department of Oral Sciences,1 Department of Stomatology, School of Dentistry, University of Otago, Dunedin 9001, New Zealand2

Received 6 November 2003/ Returned for modification 20 January 2004/ Accepted 30 January 2004

The feasibility of detecting Candida albicans mRNA in formalin-fixed paraffin-embedded archival human histopathology specimens by reverse transcription-PCR (RT-PCR) was investigated. RT with gene-specific primers was used to detect five single-copy C. albicans gene transcripts, including those of two housekeeping genes, in oral candidiasis samples up to 8 years of age.

* Corresponding author. Mailing address: Department of Oral Sciences, School of Dentistry, University of Otago, P.O. Box 647, Dunedin, New Zealand. Phone: 64 3 479 7081. Fax: 64 3 479 7078. E-mail: richard.cannon{at}stonebow.otago.ac.nz.

Journal of Clinical Microbiology, May 2004, p. 2275-2278, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2275-2278.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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