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Journal of Clinical Microbiology, May 2004, p. 2282-2284, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2282-2284.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of the Vitek-1 and Vitek-2 Systems for Detection of Constitutive and Inducible Macrolide Resistance in Group B Streptococci

Patrick Tang,1,2 Peter Ng,1 Ming Lum,1 Martin Skulnick,1 Glen W. Small,1 Donald E. Low,1,2 Alicia Sarabia,3 Tony Mazzulli,1,2 Katherine Wong,1 Andrew E. Simor,2,4 and Barbara M. Willey1*

Department of Microbiology, Toronto Medical Laboratories and Mount Sinai Hospital,1 University of Toronto,2 MDS Laboratories Inc.,3 Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario, Canada4

Received 30 November 2003/ Returned for modification 20 January 2004/ Accepted 10 February 2004

A prospective study of erythromycin and clindamycin resistance was performed with 304 consecutive group B streptococci (GBS) isolates. According to two automated susceptibility testing systems, Vitek-1 and Vitek-2, and double-disk agar diffusion, 79.9% were susceptible to both erythromycin and clindamycin. However, for macrolide-lincosamide-streptogramin B-inducible isolates, the accuracies of the Vitek-1 and Vitek-2 systems were 5.6 and 94.4%. In light of these results, we recommend that GBS be routinely tested using the double-disk diffusion method.

* Corresponding author. Mailing address: Department of Microbiology, Mount Sinai Hospital, Room 1460, 600 University Ave., Toronto, Ontario, Canada M5G 1X5. Phone: (416) 586-3121. Fax: (416) 586-3140. E-mail: bwilley{at}mtsinai.on.ca.

Journal of Clinical Microbiology, May 2004, p. 2282-2284, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2282-2284.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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