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Journal of Clinical Microbiology, June 2004, p. 2450-2454, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2450-2454.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular and Morphological Identification of Colletotrichum Species of Clinical Interest

Josep Cano,* Josep Guarro, and Josepa Gené

Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain

Received 18 June 2003/ Returned for modification 21 August 2003/ Accepted 25 February 2004

Colletotrichum species have caused human infections in recent years. Because of the difficulties in recognizing them in vitro, we have designed a quick and unambiguous molecular test, based on the amplification of a specific fragment of the internal transcribed spacer 1 region, to distinguish any Colletotrichum isolate from other fungi, including the common pathogenic species. Analysis of the sequences of the ribosomal DNA (rDNA) fragment showed sufficient variability to clearly separate the five species of Colletotrichum that are of clinical interest, i.e., Colletotrichum coccodes, C. crassipes, C. dematium, C. gloeosporioides, and C. graminicola. Sequencing of the D1-D2 region of the large-subunit rDNA gene also supported these results. Additionally, we reviewed the most suitable morphological characteristics for the in vitro identification of these increasingly important opportunistic fungi.

* Corresponding author. Mailing address: Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, 43201-Reus, Tarragona, Spain. Phone: 34 977759359. Fax: 34 977759322. E-mail: jfcl{at}astor.urv.es.

Journal of Clinical Microbiology, June 2004, p. 2450-2454, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2450-2454.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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