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Journal of Clinical Microbiology, June 2004, p. 2455-2460, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2455-2460.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Hepatitis B Virus Genotypes and Precore and Core Mutants in Brazilian Patients

Roberta Sitnik,1 João Renato Rebello Pinho,1,2* Dennis Armando Bertolini,2 Antonio Plinio Bernardini,1 Luiz Caetano da Silva,3 and Flair José Carrilho3

Laboratório Bioquímico Jardim Paulista,1 Serviço de Virologia, Instituto Adolfo Lutz,2 Departmento de Gastroenterologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil3

Received 27 June 2003/ Returned for modification 8 February 2004/ Accepted 2 March 2004

A method for genotyping hepatitis B virus by partial HBsAg gene sequencing with primers common to all known genotypes was developed. Mutations related to anti-HBs resistance are also detected with this method. Samples from 103 Brazilian patients were analyzed. Precore and core region of these viruses were also sequenced in 101 patients. Genotypes A, B, C, D, and F were found with frequencies of 49.5, 2.9, 13.6, 24.3, and 9.7%, respectively. Genotypes B and C were found only in Asian patients, whereas genotypes A, D, and F were more common in patients without an Asian background. Precore mutants were found in 32 (31.7%) of 101 patients, with a higher frequency in those infected with genotype D (22 of 25 [88.0%]). Analysis of nucleotide 1858 showed presence of thymine in all patients with genotypes B, C, and D and in a few patients with genotypes A (10.0%) and F (30.0%), who showed more frequently the presence of cytosine. This nucleotide was closely related to the presence of precore mutants. Mutations in the basal core promoter were found in 64 of 101 (63.4%) samples. These mutations were more frequent in patients infected with genotype F (90.0%) and less frequent in patients infected with genotype B (33.3%). Deletions in this region were found in two genotype C-infected patients.

* Corresponding author. Mailing address: Serviço de Virologia, Instituto Adolfo Lutz, Av. Brigadeiro Luiz Antonio, 4701, 01401-002 São Paulo, SP, Brazil. Phone: 55-11-3885-7955. Fax: 55-11-3885-7955. E-mail: jrrpinho{at}terra.com.br.

Journal of Clinical Microbiology, June 2004, p. 2455-2460, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2455-2460.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • VIANA, S., PARANA, R., MOREIRA, R. C., COMPRI, A. P., MACEDO, V. (2005). HIGH PREVALENCE OF HEPATITIS B VIRUS AND HEPATITIS D VIRUS IN THE WESTERN BRAZILIAN AMAZON. Am J Trop Med Hyg 73: 808-814 [Abstract] [Full Text]  


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