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Journal of Clinical Microbiology, June 2004, p. 2489-2494, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2489-2494.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Dengue Virus Serotyping Based on Envelope and Membrane and Nonstructural Protein NS1 Serotype-Specific Capture Immunoglobulin M Enzyme-Linked Immunosorbent Assays

Pei-Yun Shu,1 Li-Kuang Chen,2 Shu-Fen Chang,1 Chien-Ling Su,1 Li-Jung Chien,1 Chuan Chin,3 Ting-Hsiang Lin,1 and Jyh-Hsiung Huang1*

Division of Laboratory Research and Development, Center for Disease Control, Department of Health,1 Co-wealth Medical Science and Biotechnology, Taipei,3 Department of Emergency Medicine, Medical Center, Tzu Chi College of Medicine, Hualien, Taiwan, Republic of China2

Received 12 January 2004/ Returned for modification 7 February 2004/ Accepted 29 February 2004

Envelope and membrane (E/M) and nonstructural protein NS1 serotype-specific capture Immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) were developed to differentiate four dengue virus serotypes. A total of 93 anti-dengue virus IgM-positive serum samples collected between days 5 and 45 of illness from 59 confirmed dengue patients were analyzed. The results showed that positive serotype specificity could be identified for 86.1 and 47.6% of serum samples tested for E/M-specific IgM antibodies versus 83.3 and 42.9% of serum samples tested for NS1-specific IgM antibodies from patients with primary and secondary dengue virus infections, respectively. Dual analyses with both E/M and NS1 serotype-specific capture IgM ELISAs showed that positive serotype specificity could be correctly identified for 98.6 and 61.9% of all of the primary and secondary serum samples tested, respectively. These findings suggested that E/M and NS1 serotype-specific capture IgM ELISAs have the potential to be of use in dengue virus serotyping.

* Corresponding author. Mailing address: Division of Laboratory Research and Development, Center for Disease Control, Department of Health, 161, Kun-Yang St., Taipei, Taiwan, Republic of China. Phone: 886-2-26531374. Fax: 886-2-27883992. E-mail: jhhuang{at}cdc.gov.tw.

Journal of Clinical Microbiology, June 2004, p. 2489-2494, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2489-2494.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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