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Journal of Clinical Microbiology, June 2004, p. 2541-2547, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2541-2547.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of G10P[11] Rotaviruses Causing Acute Gastroenteritis in Neonates and Infants in Vellore, India

Miren Iturriza Gómara,1* Gagandeep Kang,2 Ajit Mammen,2 Atanu Kumar Jana,3 Mary Abraham,4 Ulrich Desselberger,5 David Brown,1 and Jim Gray1

Enteric Virus Unit, Enteric, Respiratory and Neurological Virus Laboratory, Specialist and Reference Microbiology Service Division, Health Protection Agency, London, United Kingdom,1 Department of Gastrointestinal Sciences,2 Neonatology Unit, Department of Child Health,3 Department of Clinical Virology, Christian Medical College and Hospital, Vellore 632004, India,4 Virologie Moléculaire et Structurale, UMR 2472, CNRS, 91198 Gif-sur-Yvette Cedex, France5

Received 31 October 2003/ Returned for modification 26 December 2003/ Accepted 20 February 2004

Rotavirus G10P[11] strains, which are commonly found in cattle, have frequently been associated with asymptomatic neonatal infections in India. We report the finding of G10P[11] strains associated with severe disease in neonates in Vellore, southern India. Rotavirus strains from 43 fecal samples collected from neonates with or without gastrointestinal symptoms between 1999 and 2000 were genotyped by reverse transcription-PCR. Forty-one neonates (95%) were infected with G10P[11] rotavirus strains, and 63% of the infections were in children who had gastrointestinal symptoms, including acute watery diarrhea. G10P[11] strains were also seen infecting older children with dehydrating gastroenteritis in Vellore. Characterization of the genes encoding VP7, VP4, VP6, and NSP4 of these strains revealed high sequence homology with the corresponding genes of the asymptomatic neonatal strain I321, which in turn is very closely related to bovine G10P[11] strains circulating in India. No significant differences were seen in the sequences obtained from strains infecting symptomatic neonates or children and asymptomatic neonates.

* Corresponding author. Mailing address: Enteric Virus Unit, Enteric Respiratory and Neurological Virus Laboratory, Health Protection Agency, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44 (0)20 82004400. Fax: 44 (0)20 82058195. E-mail: Miren.Iturriza{at}hpa.org.uk.

Journal of Clinical Microbiology, June 2004, p. 2541-2547, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2541-2547.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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