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Journal of Clinical Microbiology, June 2004, p. 2752-2758, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2752-2758.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Ileocolitis Associated with Anaerobiospirillum in Cats{dagger}

H. E. V. De Cock,1* S. L. Marks,2 B. A. Stacy,3 T. S. Zabka,3 J. Burkitt,3 G. Lu,4 D. J. Steffen,5 and G. E. Duhamel4,5,6*

Department of Pathology, Microbiology and Immunology,1 Department of Medicine and Epidemiology,2 Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, California 95616,3 Center for Biotechnology,4 Veterinary Diagnostic Center,5 Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska 68583-09056

Received 10 October 2003/ Returned for modification 29 January 2004/ Accepted 26 February 2004

Ileocolitis associated with spiral bacteria identified as an Anaerobiospirillum sp. was found in six cats. Two cats had acute onset of gastrointestinal signs characterized by vomiting and diarrhea in one cat and vomiting in another cat, one cat had chronic diarrhea that was refractory to medical therapy; one cat had acute onset of anorexia and lethargy, and two cats had clinical signs that were not related to the gastrointestinal tract. The presence of an Anaerobiospirillum sp. was demonstrated on the basis of ultrastructural morphology of spiral bacteria associated with intestinal lesions and PCR amplification of a genus-specific 16S rRNA gene from affected tissues from each cat. The colons of three clinically healthy cats without lesions and one cat with mild colitis not associated with spiral bacteria were negative for Anaerobiospirillum spp. in the same assay. Comparative nucleotide sequence analysis of cloned PCR products from three affected cats further suggested that the spiral bacteria were closely related to Anaerobiospirillum succiniciproducens.

* Corresponding author. Mailing address for H. E. V. De Cock: Department of Pathology, Microbiology and Immunology, University of California, One Shields Ave, Davis, CA 95616. Phone: (530) 752-1368. Fax: (530) 752-3349. E-mail: hedecock{at}ucdavis.edu. Gerald E. Duhamel, Department of Veterinary and Biological Sciences, Room 147, Veterinary Basic Science Bldg., University of Nebraska-Lincoln, Lincoln, NE 68583-0905. Phone: (402) 472-3862. Fax: (402) 472-9690. E-mail: gduhamel1{at}unl.edu. Mailing address for G. E. Duhamel: Department of Veterinary and Biological Sciences, Room 147, Veterinary Basic Science Bldg., University of Nebraska-Lincoln, Lincoln, NE 68583-0905. Phone: (402) 472-3862. Fax: (402) 472-9690. E-mail: gduhamel1{at}unl.edu.

{dagger} Published as paper no. 14193, Agriculture Research Division, Institute for Agriculture and Natural Resources, University of Nebraska, Lincoln, NE 68583.

Journal of Clinical Microbiology, June 2004, p. 2752-2758, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2752-2758.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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