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Journal of Clinical Microbiology, June 2004, p. 2759-2765, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2759-2765.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of a Recombinant Envelope Protein Subunit Antigen for Specific Serological Diagnosis of West Nile Virus Infection

David W. C. Beasley,1 Michael R. Holbrook,1 Amelia P. A. Travassos da Rosa,1 Lark Coffey,1 Anne-Sophie Carrara,1 Kathrine Phillippi-Falkenstein,2 Rudolf P. Bohm Jr.,2 Marion S. Ratterree,2 Kristy M. Lillibridge,3 George V. Ludwig,4 Jose Estrada-Franco,1 Scott C. Weaver,1 Robert B. Tesh,1 Robert E. Shope,1 and Alan D. T. Barrett1*

Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0609,1 Tulane National Primate Research Center, Tulane University, Covington, Louisiana 70433,2 UT Houston School of Public Health, Houston, Texas 77030,3 Diagnostic Systems and Virology Divisions, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-50114

Received / Returned for modification 22 October 2003/ Accepted 7 February 2004

Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.

* Corresponding author. Mailing address: Department of Pathology, UTMB, 301 University Blvd., Galveston, TX 77555-0609. Phone: (409) 772-6662. Fax: (409) 772-2500. E-mail: abarrett{at}utmb.edu.

Journal of Clinical Microbiology, June 2004, p. 2759-2765, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2759-2765.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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