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Journal of Clinical Microbiology, June 2004, p. 2810-2812, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2810-2812.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Clonality of Streptococcus pneumoniae Serotype 1 Isolates from Pediatric Patients in the United States

Blanca E. Gonzalez, Kristina G. Hulten, Sheldon L. Kaplan, Edward O. Mason Jr.,^* and the U.S. Pediatric Multicenter Pneumococcal Surveillance Study Group

Division of Infectious Diseases, Department of Pediatrics, Baylor College of Medicine, and the Infectious Disease Research Laboratory, Texas Children's Hospital, Houston, Texas

Received 15 January 2004/ Returned for modification 23 February 2004/ Accepted 16 March 2004

We compared Streptococcus pneumoniae serotype 1 isolates causing disease among children in six geographic regions of the United States to determine genetic relatedness. Genomic fingerprints were determined by repetitive element polymorphism PCR (Rep-PCR). Multilocus sequence type characterization was performed on selected isolates. Four different genomic banding patterns were identified by Rep-PCR. One profile (clone 1) was predominant and matched sequence type 227.

Contributing members of the U.S. Pediatric Multicenter Pneumococcal Surveillance Study Group are listed in Acknowledgments.

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