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Journal of Clinical Microbiology, June 2004, p. 2836-2839, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2836-2839.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of Campylobacter jejuni Multilocus Sequence Type ST-21 Clonal Complex by Single-Nucleotide Polymorphism Analysis

E. L. Best,1* A. J. Fox,2 J. A. Frost,3 and F. J. Bolton2

Campylobacter Reference Unit, Laboratory of Enteric Pathogens, Specialist and Reference Microbiology Division, Health Protection Agency, London,1 Health Protection Agency North West Laboratory, Manchester Medical Microbiology Partnership, Manchester Royal Infirmary, Manchester,2 Welsh Assembly Government, Cathays Park, Cardiff, Wales, United Kingdom3

Received 28 November 2003/ Returned for modification 27 January 2004/ Accepted 24 February 2004

Conserved single-nucleotide polymorphisms (SNPs) which characterize the allelic profile of the major epidemiological lineage ST-21 were identified from the alleles within the current Campylobacter jejuni multilocus sequence typing (MLST) database. Allelic discrimination assays were designed for the detection of SNPs, enabling rapid strain profiling for clonal complex ST-21. This method is suitable for epidemiological investigations and is complementary to full MLST.

* Corresponding author. Mailing address: Campylobacter Reference Unit, Laboratory of Enteric Pathogens, Specialist and Reference Microbiology Division, Health Protection Agency, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 0208 200 4400. Fax: 0208 9059929. E-mail: emma.best{at}hpa.org.uk.

Journal of Clinical Microbiology, June 2004, p. 2836-2839, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2836-2839.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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