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Journal of Clinical Microbiology, June 2004, p. 2840-2842, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2840-2842.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of Paraffin-Embedded Tissue for Identification of Saccharomyces cerevisiae in a Baker's Lung Nodule by Fungal PCR and Nucleotide Sequencing

Ping Ren,1 Sundara Sridhar,2 and Vishnu Chaturvedi1,3*

Mycology Laboratory, Wadsworth Center, New York State Department of Health,1 Department of Biomedical Sciences, SUNY School of Public Health, University at Albany, Albany,3 Department of Pathology, Coney Island Hospital, Brooklyn, New York2

Received 17 November 2003/ Returned for modification 16 December 2003/ Accepted 4 March 2004

A 40-year-old healthy male employed in a bakery presented with a single lung nodule and underwent investigations to rule out pulmonary carcinoma. Biopsy was positive for yeast cells, which did not match common fungal pathogens. PCR assay of paraffin-embedded tissue and nucleotide sequencing with ribosomal ITS1-ITS2 universal primers revealed the presence of Saccharomyces cerevisiae.

* Corresponding author. Mailing address: Mycology Laboratory, Wadsworth Center, New York State Department of Health, SUNY School of Public Health, University at Albany, Albany, NY 12201-2002. Phone: (518) 453-6412. Fax: (518) 486-7811. E-mail: vishnu{at}wadsworth.org.

Journal of Clinical Microbiology, June 2004, p. 2840-2842, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2840-2842.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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