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Journal of Clinical Microbiology, July 2004, p. 2902-2906, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.2902-2906.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Extended-Spectrum Beta-Lactamases Produced by Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from a Korean Nationwide Survey

Seok Hoon Jeong,1,2 Il Kwon Bae,1,2 Jung Hun Lee,3 Seung Ghyu Sohn,4 Geun Ho Kang,4 Ghil Ja Jeon,4 Young Ho Kim,4 Byeong Chul Jeong,3 and Sang Hee Lee3,4*

Department of Biological Science, Myongji University, Yongin, Kyunggido 449-728,3 Bio Technology Innovation Center, Youngdong University, Chungbuk 370-701,4 Department of Laboratory Medicine, Kosin University College of Medicine, Busan 602-702,1 Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, South Korea2

Received 14 October 2003/ Returned for modification 15 December 2003/ Accepted 29 March 2004

To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Klebsiella pneumoniae and Escherichia coli, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producing isolates were detected in every region in Korea. A total of 44.6% of the isolates produced both TEM- and SHV-type ESBLs, and 52% of ESBL-producing isolates transferred resistance to ceftazidime by transconjugation. The ESBLs were TEM-19, TEM-20, TEM-52, SHV-2a, SHV-12, and one new variant identified for the first time in Korea, namely, TEM-116. TEM-1 and SHV-12 were by far the most common variants. TEM-1, TEM-116, and SHV-12 showed a high prevalence in K. pneumoniae. Two isolates (E. coli SH16 and K. pneumoniae SV3) produced CMY-1-like beta-lactamases, which play a decisive role in resistance to cefoxitin and cefotetan, as well as TEM-type enzymes (TEM-20 and TEM-52, respectively). Using MIC patterns and DNA sequencing analysis, we postulated a possible evolution scheme among TEM-type beta-lactamases in Korea: from TEM-1 to TEM-19, from TEM-19 to TEM-20, and from TEM-20 to TEM-52.

* Corresponding author. Mailing address: Department of Biological Science, Myongji University, San 38-2 Namdong, Yongin, Kyunggido 449-728, South Korea. Phone: 82 31 330 6195. Fax: 82 31 335 8249. E-mail: sangheeLEE{at}mju.ac.kr.

Journal of Clinical Microbiology, July 2004, p. 2902-2906, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.2902-2906.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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