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Journal of Clinical Microbiology, July 2004, p. 3120-3127, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3120-3127.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Simultaneous Runs of the Bayer VERSANT HIV-1 Version 3.0 and HCV bDNA Version 3.0 Quantitative Assays on the System 340 Platform Provide Reliable Quantitation and Improved Work Flow

Tarek Elbeik,1,2* Norman Markowitz,3 Patricia Nassos,1,2 Uday Kumar,3 Scott Beringer,4 Barbara Haller,1,2 and Valerie Ng1,2

Department of Laboratory Medicine, University of California,1 Clinical Laboratory, San Francisco General Hospital,2 Professional Habitat Design, LLC, San Francisco, California,4 Division of Infectious Diseases, Henry Ford Hospital, Detroit, Michigan3

Received 11 June 2003/ Returned for modification 25 September 2003/ Accepted 24 March 2004

Branched DNA (bDNA) assays to quantify human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) consist of three distinct steps, including sample processing, hybridization, and detection, and utilize the System 340 platform for plate incubation and washing. Sample processing differs: HIV-1 from 1 ml of plasma is concentrated by high-speed centrifugation, whereas HCV plasma or serum samples are used without concentration. The first step of hybridization involves viral lysis at 63°C: HIV-1 is performed in a heat block, whereas HCV is performed in System 340. The remaining hybridization and detection steps are similar for HIV-1 and HCV and executed on System 340. In the present study, the HIV-1 bDNA assay was adapted for viral lysis in the System 340 platform. The adaptation, test method 2, includes a 20-s vortex of concentrated viral pellet and lysis working solution, transfer of viral lysate to the 96-well capture plate, and transfer to System 340 programmed for HCV assay specifications. With test method 2, specificity and quantification were within assay specifications. HCV bDNA methodology remains unchanged. Hence, an HIV-1 and an HCV bDNA can be run simultaneously on System 340. With simultaneous testing, laboratories can run full plates, as well as combinations of full and partial plates. Also, simultaneous HIV-1 and HCV bDNA permits labor consolidation and improved workflow while maintaining multitasking and rapid patient result turnaround.

* Corresponding author. Mailing address: San Francisco General Hospital, Department of Laboratory Medicine, Bldg. NH, Rm. 2M35, 1001 Potrero Ave., San Francisco, CA 94110. Phone: (415) 476-4604. Fax: (415) 206-3045. E-mail: elbeik{at}itsa.ucsf.edu.

Journal of Clinical Microbiology, July 2004, p. 3120-3127, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3120-3127.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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