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Journal of Clinical Microbiology, July 2004, p. 3272-3280, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3272-3280.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development and Clinical Evaluation of a Highly Sensitive DNA Microarray for Detection and Genotyping of Human Papillomaviruses

Tae Jeong Oh,1,{dagger} Chang Jin Kim,2,{dagger} Suk Kyung Woo,1 Tae Seung Kim,3 Dong Jun Jeong,2 Myung Soon Kim,1 Sunwoo Lee,1 Hyun Sill Cho,1 and Sungwhan An1*

Research and Development, GenomicTree, Inc., Taejon,1 Department of Pathology, College of Medicine, Soonchunhyang University, Chonan,2 Department of Pathology, College of Medicine, Yonsei University, Seoul, South Korea3

Received 22 September 2003/ Returned for modification 14 December 2003/ Accepted 15 March 2004

Human papillomavirus (HPV) has been found in cervical cancer, tonsillar cancer, and certain types of head and neck cancers. We report on a DNA microarray-based method for the simultaneous detection and typing of HPVs. The genotype spectrum discriminated by this HPV DNA microarray includes 15 high-risk HPV genotypes and 12 low-risk HPV genotypes. The HPV DNA microarray showed high degrees of specificity and reproducibility. We evaluated the performance of the HPV DNA microarray by application to three HPV-positive cell lines (HeLa, Caski, and SiHa cells) and two HPV-negative cell lines (C33A and A549 cells). The HPV DNA microarray successfully identified the known types of HPV present in the cell lines. The detection limit of the HPV DNA microarray was at least 100-fold higher than that of PCR. To assess the clinical applicability of the HPV DNA microarray, we performed the HPV genotyping assay with 73 nonmalignant and malignant samples from 39 tonsillar cancer patients. Twenty-five of the 39 (64.1%) malignant samples were positive for HPV, whereas 3 of 34 (8.8%) nonmalignant samples were positive for HPV. This result shows a preferential association of HPV with tonsillar carcinomas. The correlations of the presence of HPV with the grade of differentiation and risk factors were not significant. Our data show that the HPV DNA microarray may be useful for the diagnosis and typing of HPV in large-scale epidemiological studies.

* Corresponding author. Mailing address: Research and Development, GenomicTree, Inc., 461-6 Jonmin-dong Yusong, Taejon 305-811, South Korea. Phone: 82-42-861-4550. Fax: 82-42-861-4552. E-mail: info{at}genomictree.com.

{dagger} T.O. and C.K. contributed equally to this work.

Journal of Clinical Microbiology, July 2004, p. 3272-3280, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3272-3280.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jiang, H.-L., Zhu, H.-H., Zhou, L.-F., Chen, F., Chen, Z. (2006). Genotyping of human papillomavirus in cervical lesions by L1 consensus PCR and the Luminex xMAP system. J Med Microbiol 55: 715-720 [Abstract] [Full Text]  


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