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Journal of Clinical Microbiology, July 2004, p. 3284-3287, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3284-3287.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

LightCycler-Based Differentiation of Mycobacterium abscessus and Mycobacterium chelonae

L. Sedlacek,1 M. Rifai,2 K. Feldmann,2 and F. C. Bange1*

Department of Medical Microbiology and Hospital Epidemiology, Medical School Hannover, 30625 Hannover,1 Asklepios Fachklinik, 82131 München-Gauting, Germany2

Received 13 January 2004/ Returned for modification 1 March 2004/ Accepted 17 March 2004

In this study we introduce a rapid procedure to identify Mycobacterium abscessus (types I and II) and M. chelonae using LightCycler-based analysis of the hsp65 gene. Results from 36 clinical strains were compared with hsp65 gene restriction analysis and biochemical profiles of bacilli. As all three methods yielded identical results for each isolate, this procedure offers an excellent alternative to previously established nucleic acid amplification-based techniques for the diagnosis of mycobacterial diseases.

* Corresponding author. Mailing address: Department of Medical Microbiology and Hospital Epidemiology, Medical School Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. Phone: 49-511-532-4359. Fax: 49-511-532-4366. E-mail: bange{at}mikrobio.mh-hannover.de.

Journal of Clinical Microbiology, July 2004, p. 3284-3287, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3284-3287.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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