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Journal of Clinical Microbiology, July 2004, p. 3288-3290, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3288-3290.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detailed Protocol for Purification of Chlamydia pneumoniae Elementary Bodies

Sanghamitra Mukhopadhyay,1 Alejandra P. Clark,1 Erin D. Sullivan,1 Richard D. Miller,2 and James T. Summersgill1,2*

Division of Infectious Diseases, Department of Medicine,1 Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, Kentucky 402922

Received 13 November 2003/ Returned for modification 18 February 2004/ Accepted 16 April 2004

A detailed protocol for the growth and harvest of purified elementary bodies of Chlamydia pneumoniae is presented. This procedure utilizes a flask-to-flask passage scheme designed to achieve high bacterial titers in a short period of time.

* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Medicine, Rm. 311 B Building, 500 South Preston St., University of Louisville, Louisville, KY 40292. Phone: (502) 852-5132. Fax: (502) 852-1512. E-mail: j.summersgill{at}louisville.edu.

Journal of Clinical Microbiology, July 2004, p. 3288-3290, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3288-3290.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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