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Journal of Clinical Microbiology, July 2004, p. 3300-3302, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3300-3302.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Performance of the MagNA Pure LC Robot for Extraction of Chlamydia trachomatis and Neisseria gonorrhoeae DNA from Urine and Swab Specimens

Nicholas Dalesio,1 Vince Marsiglia,2 Andrew Quinn,1 Thomas C. Quinn,1,3 and Charlotte A. Gaydos1*

School of Medicine, Johns Hopkins University,1 Baltimore City Health Department, Baltimore, Maryland,2 National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland3

Received 13 January 2004/ Returned for modification 7 March 2004/ Accepted 21 March 2004

DNA from uncentrifuged urines (n = 195 and n = 202) and cervical swabs (n = 221 and n = 601) was extracted by the MagNA Pure LC robot and the Amplicor method to validate the robot's extraction for Chlamydia trachomatis and Neisseria gonorrhoeae testing by Roche PCR. The robot provided a highly sensitive and specific method of DNA extraction.

* Corresponding author. Mailing address: 1159 Ross Building, 720 Rutland Ave., Baltimore, MD 21205. Phone: (410) 614-0932. Fax: (410) 614-9774. E-mail: cgaydos{at}jhmi.edu.

Journal of Clinical Microbiology, July 2004, p. 3300-3302, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3300-3302.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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