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Journal of Clinical Microbiology, July 2004, p. 3309-3312, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3309-3312.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Novel Multiplex Real-Time PCR Assay for Rapid Typing of Major Staphylococcal Cassette Chromosome mec Elements

Patrice Francois,1* Gesuele Renzi,2 Didier Pittet,3 Manuela Bento,1 Daniel Lew,1,2 Stephan Harbarth,3 Pierre Vaudaux,1 and Jacques Schrenzel1,2

Genomic Research Laboratory,1 Clinical Microbiology Laboratory, Division of Infectious Diseases,2 Infection Control Program, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland3

Received 15 January 2004/ Returned for modification 9 March 2004/ Accepted 22 March 2004

We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

* Corresponding author. Mailing address: University of Geneva Hospitals, Division of Infectious Diseases/Genomic Research Laboratory, CH-1211 Geneva 14, Switzerland. Phone: (41)-22 372 9338. Fax: (41)-22 372 9830. E-mail: patrice.francois{at}genomic.ch.

Journal of Clinical Microbiology, July 2004, p. 3309-3312, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3309-3312.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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