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Journal of Clinical Microbiology, July 2004, p. 3317-3320, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3317-3320.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Real-Time PCR Assay for Detection and Genotype Differentiation of Giardia lamblia in Stool Specimens

Rebecca A. Guy,* Chongxie Xiao, and Paul A. Horgen

<[ERROR]zaff;1[ERROR]>University of Toronto at Mississauga, Mississauga, Ontario, Canada L5L 1C6

Received 9 December 2003/ Returned for modification 25 January 2004/ Accepted 21 March 2004

Real-time PCR, using dual-labeled fluorescent probes targeting the ß-giardin gene, was used to detect Giardia lamblia in human stool specimens and to discriminate between isolates from the two major genetic assemblages of G. lamblia infective to humans, assemblages A and B.

* Corresponding author. Mailing address: Department of Biology, University of Toronto at Mississauga, 3359 Mississauga Rd. N, Mississauga, Ontario, Canada L5L 1C6. Phone: (905) 828-5430. Fax: (905) 828-3792. E-mail: rguy{at}utm.utoronto.ca.

Journal of Clinical Microbiology, July 2004, p. 3317-3320, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3317-3320.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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