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Journal of Clinical Microbiology, July 2004, p. 3324-3325, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3324-3325.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Application of Oxidation-Reduction Assay for Monitoring Treatment of Patients with Pulmonary Tuberculosis

Parissa Farnia,* Foroazan Mohammadi, Mehdi Mirsaedi, Abolhasan Zia Zarife, Javad Tabatabee, Katayoon Bahadori, Muslem Bahadori, Mohammad Reza Masjedi, and Ali Akbar Velayati

National Research Institute of Tuberculosis and Lung Disease, Shaheed Beheshti University of Medical Sciences & Health Services, Darabad, Tehran 19556, Iran

Received 10 August 2003/ Returned for modification 12 October 2003/ Accepted 3 December 2003

By oxidation-reduction assay, the viability of Mycobacterium tuberculosis in sputum specimens was evaluated. The technique is based on the Alamar Blue and Malachite Green dyes, which change their color in response to M. tuberculosis growth. The method is simple, permits visual reading of results, and is applicable for laboratories with limited resources.

* Corresponding author. Mailing address: Iranian National Reference TB Laboratory, National Research Institute of Tuberculosis and Lung Disease, Shaheed Bahonar Ave., Darabad, Tehran 19556, P.O.: 19575/154, Iran. Phone: 0098212282111. Fax: 0098212285777. E-mail: pfarnia{at}hotmail.com.

Journal of Clinical Microbiology, July 2004, p. 3324-3325, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3324-3325.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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