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Journal of Clinical Microbiology, July 2004, p. 3326-3328, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3326-3328.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of a Real-Time PCR Assay Using the LightCycler System for Detection of Parvovirus B19 DNA

Richard S. Buller* and Gregory Storch

Department of Pediatrics, St. Louis Children's Hospital, Washington University School of Medicine, St. Louis, Missouri 63110

Received 14 January 2004/ Returned for modification 14 March 2004/ Accepted 8 April 2004

We evaluated the artus RealArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.

* Corresponding author. Mailing address: Department of Pediatrics, Box 8116, Washington University School of Medicine at St. Louis Children's Hospital, One Children's Place, St. Louis, MO 63110. Phone: (314) 454-6151. Fax: (314) 454-2274. E-mail: buller{at}kids.wustl.edu.

Journal of Clinical Microbiology, July 2004, p. 3326-3328, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3326-3328.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2004 by the American Society for Microbiology. All rights reserved.