This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Procop, G. W.
Right arrow Articles by Weinstein, M. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Procop, G. W.
Right arrow Articles by Weinstein, M. P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2004, p. 3333-3335, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3333-3335.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Pneumocystis jiroveci in Respiratory Specimens by Four Staining Methods

G. W. Procop,1* S. Haddad,2 J. Quinn,3 M. L. Wilson,3 N. G. Henshaw,4,5 L. B. Reller,4,5,6 R. L. Artymyshyn,2 M. T. Katanik,1 and M. P. Weinstein2,7,8

Section of Clinical Microbiology, Department of Clinical Pathology, The Cleveland Clinic Foundation, Cleveland, Ohio,1 Microbiology Laboratory,7 Departments of Medicine,8 Pathology, University of MedicineDentistry—Robert Wood Johnson Medical School, New Brunswick, New Jersey,2 Department of Pathology, Denver Health Medical Center, Denver, Colorado,3 Clinical Microbiology Laboratory,4 Departments of Pathology,5 Medicine, Duke University Medical Center, Durham, North Carolina6

Received 18 January 2004/ Returned for modification 27 February 2004/ Accepted 6 April 2004

We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain were 49.2 and 99.6%, respectively. The sensitivity and specificity of Merifluor Pneumocystis stain were 90.8 and 81.9%, respectively. Only CW and GMS had positive and negative predictive values of >90%.

* Corresponding author. Mailing address: Section of Clinical Microbiology, Department of Clinical Pathology, The Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195. Phone: (216) 444-5879. Fax: (216) 445-6984. E-mail: procopg{at}ccf.org.

Journal of Clinical Microbiology, July 2004, p. 3333-3335, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3333-3335.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huggett, J F, Taylor, M S, Kocjan, G, Evans, H E, Morris-Jones, S, Gant, V, Novak, T, Costello, A M, Zumla, A, Miller, R F (2008). Development and evaluation of a real-time PCR assay for detection of Pneumocystis jirovecii DNA in bronchoalveolar lavage fluid of HIV-infected patients. Thorax 63: 154-159 [Abstract] [Full Text]  
  • Tasaka, S., Hasegawa, N., Kobayashi, S., Yamada, W., Nishimura, T., Takeuchi, T., Ishizaka, A. (2007). Serum Indicators for the Diagnosis of Pneumocystis Pneumonia. Chest 131: 1173-1180 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»